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KMID : 0359319760160020105
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Korean Journal of Veterinary Research
1976 Volume.16 No. 2 p.105 ~ p.114
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Study on Improved Diluting Fluids for Direct Counts of Total Leukocyte , Eosinophil and Other Leukocytes
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Abstract
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The study was conducted for an attempt to improve the diluting fluid for total leukocyte count, and to prepare a multipurpose diluting fluid for concurrent direct counts of total leukocytes, eosinophils and the other leukocyles.
Through the experiment, two better fluids for total leukocyte count of blood of human, bovine, swine, canine and rabbit were selected, and which conserved cell morphology of leukocytes better than Turk-solution. Each formula of two fluids were given as under R I and R II.
Formula of multipurpose diluting fluid selected in the experiment was given as under III. With this fluid, direct counts of total leukocytes, eosinophils and probably basophils of blood of human, bovine and swine were practicable concurrently in the same counting chamber of a hemocytometer.
In this fluid, eosinophils were stained red in the part of eosinophilic granules and blue in other part of cell, and basophils were stained dark blue like as a lump of black granules, staining three other leukocytes faint blue. Eosinophils of canine blood were not so enough red those in other animal and human and eosinophils of rabbit blood were not distinguishable from pseudoeosinophils in this fluid.
R 1. Normal saline solution…100㎖
Glacial acetic acid…1∼2 ㎖
1% genital violet aqueous solution…1㎖
Mix and filter
R Ⅱ. Distilled water…90 ㎖
Propylene glycol…10 ㎖
1% gentian violet aqueous solution…1 ㎖
Mix well and filter
Ⅲ. Propylene glycol…50㎖
Distilled water…40㎖
1% Phloxine aqueous solution…5㎖
1% methylene blue in methyl alcohol…5㎖
Mix thoroughly, filter and stopper tightly
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